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488 nm laser

Laser Focus World - January 2015

Laser Focus World - January 2015

…of lengths of DNA and/or to analyze longer cut strands. FIGURE 2. Gated STED (gSTED) imaging with a 577 nm OPSL. Scanning confocal and gSTED images of a) 40 nm fluorescent yellow-green beads and b) microtubule in mammalian cells immunostained with Alexa 488. Spatial resolution in A is 50 nm.

Laser Focus World - August 2016

Laser Focus World - August 2016

…water-cooled argon laser. When diode-pumped, solid-state (DPSS) lasers were first introduced, these looked like a potential alternative. However, most of the commercially available reagents for cytometry required excitation at 488 nm, and the DPSS frequency-doubled output of 532 nm was only…

Laser Focus World - August 2016

Laser Focus World - August 2016

…water-cooled argon laser. When diode-pumped, solid-state (DPSS) lasers were first introduced, these looked like a potential alternative. However, most of the commercially available reagents for cytometry required excitation at 488 nm, and the DPSS frequency-doubled output of 532 nm was only…

Laser Focus World - August 2016

Laser Focus World - August 2016

lasers, with 488 nm excitable dyes a central focus. Fluorescein isothiocyanate (FITC) and phycoerythrin (PE) attained ubiquity in nearly every flow cytometry lab as a result. As a pioneer in argon ion lasers since 1969, flow cytometry became one of Coherent's first major commercial successes. Laser

Laser Focus World - January 2015

Laser Focus World - January 2015

…also critical, as a laser problem will ruin the entire data run. Preferred laser wavelengths are still dominated by argon ion and DPSS legacy wavelengths (488, 514, 532 nm). But the wavelength scalability of OPSL technology is creating interest in new wavelengths, such as 640 nm. In particular, the…

Laser Focus World - January 2015

Laser Focus World - January 2015

…also critical, as a laser problem will ruin the entire data run. Preferred laser wavelengths are still dominated by argon ion and DPSS legacy wavelengths (488, 514, 532 nm). But the wavelength scalability of OPSL technology is creating interest in new wavelengths, such as 640 nm. In particular, the…

Laser Focus World - August 2017

Laser Focus World - August 2017

…variety of laser wavelengths used in microscopy, this approach by necessity relied on using standalone smart lasers coupled into a central module. However, in flow cytometry, the laser wavelengths are very standard--most multiwavelength instruments use 405, 488, and 640 nm, with 561 nm sometimes…

Laser Focus World - August 2017

Laser Focus World - August 2017

…sources. To do this, the instrument needs to know whether fluorescence detected at, say, 550 nm is because of excitation at 405 or 488 nm. The most common way to achieve this is to arrange the focused laser beams as a staggered sequence of elliptical foci aligned with the flow stream, as shown in…

Lasers | Photonics Handbook®

Lasers | Photonics Handbook®

lasers can be designed to operate at virtually any visible wavelength, at last freeing applications from the restrictions of limited legacywavelength choices (i.e., 488 and 514 nm from argon-ion lasers and 532 nm from frequency-doubled YAG lasers). Indeed, OPSLs represent a paradigm shift in lasers

Lasers | Photonics Handbook®

Lasers | Photonics Handbook®

lasers can be designed to operate at virtually any visible wavelength, at last freeing applications from the restrictions of limited legacywavelength choices (i.e., 488 and 514 nm from argon-ion lasers and 532 nm from frequency-doubled YAG lasers). Indeed, OPSLs represent a paradigm shift in lasers

Advances in Diode Lasers and OPSLs

Advances in Diode Lasers and OPSLs

…monitoring, metrology and scientific research. Table. 3: Operating costs for various laser types for an operation time of 10,000 hours. OPSL 488 nm Argon Ion 488 nm Laser Diode 445 nm HeCd 442 nm OPSL 355 nm Argon Ion UV Power class (mW) 50 50 50 50 100 100 Electricity costs (0.19C/kWh) 100 1,900…

Advances in Diode Lasers and OPSLs

Advances in Diode Lasers and OPSLs

…monitoring, metrology and scientific research. Table. 3: Operating costs for various laser types for an operation time of 10,000 hours. OPSL 488 nm Argon Ion 488 nm Laser Diode 445 nm HeCd 442 nm OPSL 355 nm Argon Ion UV Power class (mW) 50 50 50 50 100 100 Electricity costs (0.19C/kWh) 100 1,900…

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