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488 nm laser

Lasers | Photonics Handbook®

Lasers | Photonics Handbook®

lasers can be designed to operate at virtually any visible wavelength, at last freeing applications from the restrictions of limited legacywavelength choices (i.e., 488 and 514 nm from argon-ion lasers and 532 nm from frequency-doubled YAG lasers). Indeed, OPSLs represent a paradigm shift in lasers

Laser Focus World - January 2015

Laser Focus World - January 2015

…also critical, as a laser problem will ruin the entire data run. Preferred laser wavelengths are still dominated by argon ion and DPSS legacy wavelengths (488, 514, 532 nm). But the wavelength scalability of OPSL technology is creating interest in new wavelengths, such as 640 nm. In particular, the…

Advances in Diode Lasers and OPSLs

Advances in Diode Lasers and OPSLs

…provides power scalability, wavelength flexibility and excellent laser parameters (beam quality, power stability, noise). Coherent pioneered the commercial use of OPSL technology starting in 2001 with 20 mW power 488 nm lasers under the brand name SapphireTM (see Figure 4). The Sapphire brand…

Laser Focus World - January 2015

Laser Focus World - January 2015

…of lengths of DNA and/or to analyze longer cut strands. FIGURE 2. Gated STED (gSTED) imaging with a 577 nm OPSL. Scanning confocal and gSTED images of a) 40 nm fluorescent yellow-green beads and b) microtubule in mammalian cells immunostained with Alexa 488. Spatial resolution in A is 50 nm.

Laser Focus World - January 2015

Laser Focus World - January 2015

…of lengths of DNA and/or to analyze longer cut strands. FIGURE 2. Gated STED (gSTED) imaging with a 577 nm OPSL. Scanning confocal and gSTED images of a) 40 nm fluorescent yellow-green beads and b) microtubule in mammalian cells immunostained with Alexa 488. Spatial resolution in A is 50 nm.

Laser Focus World - January 2015

Laser Focus World - January 2015

…of lengths of DNA and/or to analyze longer cut strands. FIGURE 2. Gated STED (gSTED) imaging with a 577 nm OPSL. Scanning confocal and gSTED images of a) 40 nm fluorescent yellow-green beads and b) microtubule in mammalian cells immunostained with Alexa 488. Spatial resolution in A is 50 nm.

Laser Focus World - January 2015

Laser Focus World - January 2015

…also critical, as a laser problem will ruin the entire data run. Preferred laser wavelengths are still dominated by argon ion and DPSS legacy wavelengths (488, 514, 532 nm). But the wavelength scalability of OPSL technology is creating interest in new wavelengths, such as 640 nm. In particular, the…

Laser Focus World - January 2015

Laser Focus World - January 2015

…also critical, as a laser problem will ruin the entire data run. Preferred laser wavelengths are still dominated by argon ion and DPSS legacy wavelengths (488, 514, 532 nm). But the wavelength scalability of OPSL technology is creating interest in new wavelengths, such as 640 nm. In particular, the…

Laser Focus World - August 2016

Laser Focus World - August 2016

…water-cooled argon laser. When diode-pumped, solid-state (DPSS) lasers were first introduced, these looked like a potential alternative. However, most of the commercially available reagents for cytometry required excitation at 488 nm, and the DPSS frequency-doubled output of 532 nm was only…

Laser Focus World - August 2016

Laser Focus World - August 2016

…water-cooled argon laser. When diode-pumped, solid-state (DPSS) lasers were first introduced, these looked like a potential alternative. However, most of the commercially available reagents for cytometry required excitation at 488 nm, and the DPSS frequency-doubled output of 532 nm was only…

Laser Focus World - August 2016

Laser Focus World - August 2016

lasers, with 488 nm excitable dyes a central focus. Fluorescein isothiocyanate (FITC) and phycoerythrin (PE) attained ubiquity in nearly every flow cytometry lab as a result. As a pioneer in argon ion lasers since 1969, flow cytometry became one of Coherent's first major commercial successes. Laser

Laser Focus World - August 2016

Laser Focus World - August 2016

lasers, with 488 nm excitable dyes a central focus. Fluorescein isothiocyanate (FITC) and phycoerythrin (PE) attained ubiquity in nearly every flow cytometry lab as a result. As a pioneer in argon ion lasers since 1969, flow cytometry became one of Coherent's first major commercial successes. Laser

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