milliwatts at the laser output, based on
the efficiency of typical microscope illumination and scanning optics.
Some applications need more power.
For instance, the newer scanning-disk microscopes support faster frame rates by
imaging multiple focal spots in the sample…
…reassembled into a three-dimensional picture. The microscope is confocal because the objective lens is used both to illuminate the sample and to image it. It is a scanning optical microscope because only one point of the sample is illuminated at a time. As the sample is scanned, the image is built…
…return light will be at a shorter wavelength (blue shifted). The object can be either be solid or atmospheric - the atmosphere contains many microscopic dust and aerosol particles that are carried by the wind. These are the object of interest, as they are small and light enough to move at the true…
…Inspection? Laser inspection can be divided into two segments. One is the quality control of microscopically generated features and the determination of contamination in the semiconductor field. Microscopic laser inspection uses scattering, absorption and ultrasonic techniques to determine and…
…in the mouse lung.
BiosafetyCabinets for multiphoton microscope andTi-Sapphire laser components. The instrumentation necessary to perform MPM consists in a microscope equipped with optics to illuminate the specimen
and guide reflected light into the detectors, which is…
about combining confocal fluorescence
microscopy information with data from
the atomic force microscope (AFM) and
reflection electron microscope (REM).
Historically, these have been quite distinct disciplines often used in isolation.
Today, more and more…
…localization, based on the point spread
function of the microscope). Repetitive on/off switching brings up different
random subsets in subsequent camera
images for single-molecule localization. After many cycles, the microscope
computer assembles all the idealized point
…mechanisms for this on/ off photoswitching, optical nanoscopy methods can be loosely divided into two groups: those that directly improve microscope effective spatial resolution by deterministic photoswitching in space and time, and those that achieve the higher resolution by (random) stochastically…
…noise imaging, fast response time and fine spatial
sampling (8x8µm pitch). The broadband UV fluorescent lamp provided adequate and uniform illumination across the
small 25x25 mm sample region which in turn allowed the quantum dots to fluoresce in the visible. A total of 6 different
…least 60 min for equilibration, samples were continuously illuminated using a set of green-light LEDs with an emission maximum of 520 nm.
Additional UV light application was performed with a set of 362 nm LEDs. Singleturnover illumination was performed using a 10-Hz pulsed Nd:YAG Powerlite
They are using a tunable OPA (Coherent Opera pumped by a Monaco Yb
amplifier) together with a multiphoton
microscope from Sutter Instruments
modified by replacing the scanning tube
optics with lenses optimized for 1300-nm
transmission. Their current…
…these proteins changes the cation
(usually Ca2+) gradient, and hence the
electrical gradient, across cellular membranes, targeted local illumination can
stimulate or silence activity in the target
neuron(s). This soon led to the adoption of
channelrhodopsins (e.g., ChR1 and…