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Pump Probe

Bio Photonics - February / March 2015

Bio Photonics - February / March 2015

…transient
local Ca2+ ion concentration - is probed
simultaneously by scanning the sample,
usually with a second wavelength.
Dual-wavelength output
Owing to the growing range of calcium
indicators and optogenetics probes, the
choice of optimal wavelengths is…

Europhotonics - Spring 2016

Europhotonics - Spring 2016

…shows a view into the machining space. The lower Y-axis slide carries a turn and
swivel unit with the workpiece. It is spanned by the XZ-axes gantry where a spindle for micromilling and a co-linear touch probe measuring system are attached to the Z-slide. Courtesy of
Kugler GmbH.

Bio Photonics - July 2018

Bio Photonics - July 2018

…studies of the formation and function of lymphatic vessels in lab mice. The researchers use an arsenal of fluorescent microscopy techniques to probe these vessels and unravel the genetic control of formation, function, and repair. Multiphoton microscopy, often using triple-transgenic mice, is…

EU Photonics Talk Nobel Laureate Haensch.pdf

EU Photonics Talk Nobel Laureate Haensch.pdf

…neon lasers, I secured a NATO post-doc position with Professor Art Schawlow at Stanford University. Fortunately, I had witnessed a dye laser being pumped by a commercial nitrogen laser when I visited Bell Labs on my way to California. I persuaded Art that we should acquire a nitrogen laser, and that…

Ted Haensch  Sharing His Unique Perspective on Lasers

Ted Haensch Sharing His Unique Perspective on Lasers

…neon lasers, I secured a NATO post-doc position with Professor Art Schawlow at Stanford University. Fortunately, I had witnessed a dye laser being pumped by a commercial nitrogen laser when I visited Bell Labs on my way to California. I persuaded Art that we should acquire a nitrogen laser, and that…

Photonics Spectra - November 2016

Photonics Spectra - November 2016

…Optogenetics involves the use of light-sensitive opsins -- a special class of
membrane-embedded proteins. Opsins are switchable channels or pumps for specific
cations or anions whose on or off state is determined by irradiation with light of an appropriate wavelength. It has been known…

Optogenetics Relies on Multiphoton Microscopy | Imaging & Microscopy - Research, Development, Production

Optogenetics Relies on Multiphoton Microscopy | Imaging & Microscopy - Research, Development, Production

…available power from the combination of the OPO and its Ti:S pump is about 1 watt.
Trends in advanced optogenetics and optical physiology require higher power for several reasons. First, the emission of fluorescent
probes (when not saturated) is proportional to the laser peak power times…

COHR ChameleonDiscovery TPC DS 1117 5.pdf

COHR ChameleonDiscovery TPC DS 1117 5.pdf

…of 660 nm to 1320 nm, high power and short pulses delivered right to the sample plane enables deep in-vivo excitation of all popular fluorescent probes, like eGFP and YFP. The long wavelength capabilities address perfectly the ongoing developments in RFPs and redshifted calcium indicators. High peak…

Bio Photonics - April 2015

Bio Photonics - April 2015


be a key technique for successful translation along this path, because it avoids
the use of fluorophores and genetically
expressed probes that, of course, cannot
be used in humans.
Summary
Laser-based microscopy continues to
be one of the most valuable tools in the…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…tweaking and re-optimization by a highly skilled
laser expert. Then came Ti:sapphire lasers,
pumped first by ion lasers, then by DPSS
lasers, and then by optically pumped semiconductor lasers (OPSLs). With each generation, the lasers became smaller, simpler,
and more reliable.…