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Three-photon

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…(among which are the mFruit series). Three-photon excitation is now becoming more popular because it allows to excite at 1 micron or longer wavelengths some of the probes that in two-photon regime would require 700 - 800 nm excitation light. Although three-photon excitation requires more power, the…

whitepaper next gen mpe v3.pdf

whitepaper next gen mpe v3.pdf

A Coherent Whitepaper February 22, 2017 www.coherent.com | tech.sales@coherent.com | 800-527-3786 | 408-764-4983 Power Scalability. In optogenetics, a state of the art in-vivo experiment might involve simultaneously studying (activating and imaging) a few tens of neurons in a mouse cortex. However…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…(among which are the mFruit series). Three-photon excitation is now becoming more popular because it allows to excite at 1 micron or longer wavelengths some of the probes that in two-photon regime would require 700 - 800 nm excitation light. Although three-photon excitation requires more power, the…

Life Sciences and Medical Applications

Life Sciences and Medical Applications

DNA Sequencing - Several high-throughput techniques rely on laser fluorescence. DNA sequencing involves the use of automated and miniaturized biochemical techniques to determine the fundamental base sequence (ACGT) of strands of DNA, chromosomes and even the entire genome. DNA sequencing is…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…(among which are the mFruit series). Three-photon excitation is now becoming more popular because it allows to excite at 1 micron or longer wavelengths some of the probes that in two-photon regime would require 700 - 800 nm excitation light. Although three-photon excitation requires more power, the…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

BRAIN-2017-JTu4A-14.pdf

BRAIN-2017-JTu4A-14.pdf

…Wise, F.W., Schaffer, C., and Xu, C. "In vivo three-photon microscopy of subcortical structures of an intact mouse brain." Nat. Phot. 7, 205 - 209 (2013). [7] Horton, N.G., Wang, K., Kobat, D., Wise, F.W., and Xu, C. "In vivo deep penetration three-photon imaging of mouse brain through an unthinned,…

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