Search Results

Refine

Result Type

1 - 12 of 14 Products for 'Three-photon' Page 1 of 2 |

Three-photon

Photonics Spectra - March 2018

Photonics Spectra - March 2018

…1300-nm window is ideal for threephoton excitation of transgenic probes
based on green fluorescent proteins, and
the 1700-nm wavelength is a good match
for three-photon excitation of long wavelength probes based on red fluorescent
proteins. Three-photon imaging also offers the…

Bio Photonics - April 2018

Bio Photonics - April 2018

…replacing the scanning tube
optics with lenses optimized for 1300-nm
transmission. Their current investigations
primarily involve three-photon imaging
of GCaMP fluorescence at 1300 nm. The
fluorescence of the genetically encoded
GCaMP protein increases in the presence…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…mFruit series).
Three-photon excitation is now
becoming more popular because it allows to excite at 1 micron or longer
wavelengths some of the probes that
in two-photon regime would require
700 - 800 nm excitation light. Although
three-photon excitation requires more…

Photonics Spectra - November 2016

Photonics Spectra - November 2016

November 2016 Photonics Spectra 43
Small lab mammals such as mice can
be genetically modified so that specific
opsins are produced in their neurons, or
even selectively in only certain types of
neurons, thus enabling optogenetic studies
of live animals. Specifically,…

Bio Photonics - February / March 2015

Bio Photonics - February / March 2015


of mFruits, together with a longer tunable
wavelength (e.g., 1250 nm) for second- or
third-harmonic generation, or even threephoton excitation of some green-emitting
fluorophores.
Another group of imaging techniques
requires two different ultrafast wavelengths…

Chameleon-Vision-S-The-Next-Generation-of-One-Box-Ti-Sapphire-Lasers.pdf

Chameleon-Vision-S-The-Next-Generation-of-One-Box-Ti-Sapphire-Lasers.pdf

…excitation, the
fluorescence signal depends on the square of the
excitation power, while some damage mechanisms are
the result of three-photon absorption, possibly through
an intermediate metastable step.
Signal intensity (Ppeak
)2 x pulsewidth = Pave
x Ppeak

Chameleon Vision S whitepaper.pdf

Chameleon Vision S whitepaper.pdf

…I tech.sales@coherent.com I (800) 527-3786 I (408) 764-4983 5
excitation power while some damage mechanisms are
the results of three-photon absorption, possibly through
an intermediate metastable step.
Signal intensity (Ppeak
)2 x pulsewidth = Pave
x Ppeak

whitepaper next gen mpe v3.pdf

whitepaper next gen mpe v3.pdf

A Coherent Whitepaper February 22, 2017
www.coherent.com | tech.sales@coherent.com | 800-527-3786 | 408-764-4983
Power Scalability. In optogenetics, a state of the art in-vivo experiment might involve
simultaneously studying (activating and imaging) a few tens of neurons in a mouse…

Bio Photonics - July 2018

Bio Photonics - July 2018

…cavity, or
even tumor tissue samples from biopsies,
we sometimes need a penetration depth of
hundreds of microns."
Two- and three-photon excitation of
fluorescence using a tightly focused NIR
ultrafast laser presents several advantages. Fluorescence is only excited at…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Optogenetics Relies on Multiphoton Microscopy | Imaging & Microscopy - Research, Development, Production

Optogenetics Relies on Multiphoton Microscopy | Imaging & Microscopy - Research, Development, Production

2SWRJHQHWLFV5HOLHVRQ0XOWLSKRWRQ0LFURVFRS\ Jan. 22, 2015 Fig. 1: Multiphoton microscopy provides inherent three-dimensional resolution. With conventional (single photon) excitation of samples, fluorescence is excited along the entire beam path. With multiphoton absorption, fluorescence is only…