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Related Categories: Ytterbium | Acousto Optic Modulator

Three-photon

whitepaper next gen mpe v3.pdf

whitepaper next gen mpe v3.pdf

A Coherent Whitepaper February 22, 2017 www.coherent.com | tech.sales@coherent.com | 800-527-3786 | 408-764-4983 Power Scalability. In optogenetics, a state of the art in-vivo experiment might involve simultaneously studying (activating and imaging) a few tens of neurons in a mouse cortex. However…

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…(among which are the mFruit series). Three-photon excitation is now becoming more popular because it allows to excite at 1 micron or longer wavelengths some of the probes that in two-photon regime would require 700 - 800 nm excitation light. Although three-photon excitation requires more power, the…

Chameleon-Vision-S-The-Next-Generation-of-One-Box-Ti-Sapphire-Lasers.pdf

Chameleon-Vision-S-The-Next-Generation-of-One-Box-Ti-Sapphire-Lasers.pdf

…two-photon excitation, the fluorescence signal depends on the square of the excitation power, while some damage mechanisms are the result of three-photon absorption, possibly through an intermediate metastable step. Signal intensity (Ppeak)2 x pulsewidth = Pave x Ppeak whereas Damage probability…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…(among which are the mFruit series). Three-photon excitation is now becoming more popular because it allows to excite at 1 micron or longer wavelengths some of the probes that in two-photon regime would require 700 - 800 nm excitation light. Although three-photon excitation requires more power, the…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…(among which are the mFruit series). Three-photon excitation is now becoming more popular because it allows to excite at 1 micron or longer wavelengths some of the probes that in two-photon regime would require 700 - 800 nm excitation light. Although three-photon excitation requires more power, the…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

BRAIN-2017-JTu4A-14.pdf

BRAIN-2017-JTu4A-14.pdf

…Wise, F.W., Schaffer, C., and Xu, C. "In vivo three-photon microscopy of subcortical structures of an intact mouse brain." Nat. Phot. 7, 205 - 209 (2013). [7] Horton, N.G., Wang, K., Kobat, D., Wise, F.W., and Xu, C. "In vivo deep penetration three-photon imaging of mouse brain through an unthinned,…

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