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Three-photon

Photonics Spectra - March 2018

Photonics Spectra - March 2018

…1300-nm window is ideal for threephoton excitation of transgenic probes
based on green fluorescent proteins, and
the 1700-nm wavelength is a good match
for three-photon excitation of long wavelength probes based on red fluorescent
proteins. Three-photon imaging also offers the…

Lasers for Optogenetics and Multimodal Microscopy

Lasers for Optogenetics and Multimodal Microscopy

…mFruit series).
Three-photon excitation is now
becoming more popular because it allows to excite at 1 micron or longer
wavelengths some of the probes that
in two-photon regime would require
700 - 800 nm excitation light. Although
three-photon excitation requires more…

Bio Photonics - July 2018

Bio Photonics - July 2018

…cavity, or
even tumor tissue samples from biopsies,
we sometimes need a penetration depth of
hundreds of microns."
Two- and three-photon excitation of
fluorescence using a tightly focused NIR
ultrafast laser presents several advantages. Fluorescence is only excited at…

whitepaper next gen mpe v3.pdf

whitepaper next gen mpe v3.pdf

A Coherent Whitepaper February 22, 2017
www.coherent.com | tech.sales@coherent.com | 800-527-3786 | 408-764-4983
Power Scalability. In optogenetics, a state of the art in-vivo experiment might involve
simultaneously studying (activating and imaging) a few tens of neurons in a mouse…

Bio Photonics - April 2018

Bio Photonics - April 2018

…replacing the scanning tube
optics with lenses optimized for 1300-nm
transmission. Their current investigations
primarily involve three-photon imaging
of GCaMP fluorescence at 1300 nm. The
fluorescence of the genetically encoded
GCaMP protein increases in the presence…

Life Sciences and Medical Applications

Life Sciences and Medical Applications

DNA Sequencing - Several high-throughput techniques rely on laser fluorescence. DNA sequencing involves the use of automated and miniaturized biochemical techniques to determine the fundamental base sequence (ACGT) of strands of DNA, chromosomes and even the entire genome. DNA sequencing is…

Bio Optics World - May/June 2014

Bio Optics World - May/June 2014

…beam on the image plane (x-y) and moving the sample, or the objective, in the z direction. Common examples of nonlinear imaging include two- and threephoton excitation of fluorophores (MPE), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and third-harmonic generation…

Bio Photonics - March 2014

Bio Photonics - March 2014

…pharmaceuticals, where the SHG signal occurs only in the presence of noncentrosymmetric-ordered crystals. Others are based on three-photon effects including three-photon excitation, THG (third-harmonic generation) and CARS (coherent anti-Stokes Raman scattering). "One specific advantage in…

Optogenetics Relies on Multiphoton Microscopy | Imaging & Microscopy - Research, Development, Production

Optogenetics Relies on Multiphoton Microscopy | Imaging & Microscopy - Research, Development, Production

2SWRJHQHWLFV5HOLHVRQ0XOWLSKRWRQ0LFURVFRS\ Jan. 22, 2015 Fig. 1: Multiphoton microscopy provides inherent three-dimensional resolution. With conventional (single photon) excitation of samples, fluorescence is excited along the entire beam path. With multiphoton absorption, fluorescence is only…

Europhotonics - Autumn 2017

Europhotonics - Autumn 2017

…applications space is changing and so must the optics and lasers that support these methods." Among the most dynamic areas are optogenetics and three-photon imaging. For these methods, researchers are pushing for longer wavelengths (and higher powers) beyond 1 µm, which has required new lasers with…

Chameleon-Vision-S-The-Next-Generation-of-One-Box-Ti-Sapphire-Lasers.pdf

Chameleon-Vision-S-The-Next-Generation-of-One-Box-Ti-Sapphire-Lasers.pdf

…excitation, the
fluorescence signal depends on the square of the
excitation power, while some damage mechanisms are
the result of three-photon absorption, possibly through
an intermediate metastable step.
Signal intensity (Ppeak
)2 x pulsewidth = Pave
x Ppeak

Chameleon Vision S whitepaper.pdf

Chameleon Vision S whitepaper.pdf

…I tech.sales@coherent.com I (800) 527-3786 I (408) 764-4983 5
excitation power while some damage mechanisms are
the results of three-photon absorption, possibly through
an intermediate metastable step.
Signal intensity (Ppeak
)2 x pulsewidth = Pave
x Ppeak